Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 704-709, 2011.
Article
in English
| WPRIM
| ID: wpr-328429
ABSTRACT
<p><b>OBJECTIVE</b>To study the characteristics of lymphocyte nuclear factor kappa B (NF-κB) signal transduction kinase-related molecular mRNA differential expressions at various month age segments in aging process and the intervening effect of Epimedium flavonoids (EF) on it.</p><p><b>METHODS</b>Sixty SD rats were divided into six groups, according to animals' age, i.e., the 3 days (d) group, the 4 months (m) group, the 10 m group, the 18 m group, the 27 m group, and the 27 m+EF group. RNA was extracted from separated splenic lymphocytes. Adopting NF-κB signal path functional genome oligonucleotide gene-chip (128 related genes), the integral characteristics and differences of NF-κB signal transduction kinase-related mRNA expressions were determined, and the intervening effect of EF was examined.</p><p><b>RESULTS</b>The mean level of the NF-κB signal transduction kinase-related mRNA expressions in rats' splenic lymphocytes lowered with aging; the highest expression was presented at 3 d after birth, and then, it lowered gradually, with the lowest level at 18 m or 27 m. After EF intervention, the expression level was raised to the 10-18 m level in the aged rats.</p><p><b>CONCLUSION</b>The changing rules of lymphocyte NF-κB-signal-transduction-kinase-related mRNA expressions in various stages of aging are helpful for selecting the well time for preventing and intervening aging, and will also give a hint to the molecular index for assessment of senility retarding researches.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Flavonoids
/
Aging
/
RNA, Messenger
/
Lymphocytes
/
Signal Transduction
/
Chemistry
/
Gene Expression Regulation
/
Rats, Sprague-Dawley
/
Protein Serine-Threonine Kinases
Limits:
Animals
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS