Dahuang zhechong pill containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-extracellular regulated kinase 1/2 signaling pathway / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 371-377, 2012.
Article
in English
| WPRIM
| ID: wpr-328506
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effects of dahuang zhechong pill ( DHZCP) on the cell cycle and the related signal pathways in vascular smooth muscle cells (VSMCs) stimulated by platelet-derived growth factor (PDGF) with the method of serum pharmacology.</p><p><b>METHODS</b>DNA synthesis in VSMCs was examined by detecting 5'-bromo-2'-deoxyuridine incorporation with the immunocytochemical method. The cycle of VSMCs was evaluated with flow cytometry. Expressions of cyclin D1, p27, protein kinase Cα (PKCα), and phosphorylated extracellular signal regulated kinase 1/2 (ERK1/2) were quantified by Western blot method.</p><p><b>RESULTS</b>DHZCP containing serum significantly inhibited DNA synthesis of PDGF-stimulated VSMCs, arrested the cells in G G(1) phase, modulated the protein expressions of cyclin D D(1) and p27, and suppressed the activation of PKCα and ERK1/2.</p><p><b>CONCLUSION</b>DHZCP containing serum inhibits VSMCs proliferation via modulating the expressions of cell cycle proteins to arrest the cell in G G(1) phase, which is attributed to, at least in part, suppressing PKCα-ERK1/2 signaling in VSMCs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta, Thoracic
/
Pharmacology
/
Physiology
/
DNA
/
Platelet-Derived Growth Factor
/
Drugs, Chinese Herbal
/
Blood Proteins
/
Cells, Cultured
/
G1 Phase
/
Rats, Sprague-Dawley
Limits:
Animals
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2012
Type:
Article
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