Induction of apoptosis of leukemic tumor cells in mouse model with G-quadruplex ligand Tel03 / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 57-60, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-328572
ABSTRACT
This study was aimed to investigate the anti-leukemia activity of Tel03 in vivo. The K562 xenografted leukemia model was established and mice were divided randomly into three groups. Mice of different group were treated with PBS (control), 5 mg/kg Tel03 or 15 mg/kg Tel03 (ip, twice a week) respectively. Tumor volume, body weight and other behavior were observed regularly. Cell apoptosis was detected with TUNEL assay and the expression levels of Bcl-2 and Bax were detected by Western blot. The results indicated that Tel03 exerted anti-leukemia activity in mouse model. Tel03 significantly reduced tumor volume in Tel03-treated group compared with control. In addition, 5 mg/kg Tel03 induced cell apoptosis without exerting apparent toxicity in mice. After Tel03 treatment, the expression of Bcl-2 was inhibited, however, the expression of Bax was up-regulated. It is concluded that G-quadruplex ligand Tel03 can induce cell apoptosis in leukemia mouse model, and this agent may be a potential anticancer drug.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Proto-Oncogene Proteins
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
K562 Cells
/
Xenograft Model Antitumor Assays
/
Bcl-2-Associated X Protein
/
G-Quadruplexes
/
Metabolism
/
Mice, Inbred BALB C
/
Mice, Nude
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2010
Type:
Article
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