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Expression and activity of glycosylphosphatidylinositol-specific phospholipase d mRNA in bone marrow mononuclear cells isolated from patient with acute myeloid leukemia and their significance / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 15-18, 2010.
Article in Chinese | WPRIM | ID: wpr-328581
ABSTRACT
This study was purposed to investigate the expression and significance of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) in bone marrow mononuclear cells (BMMNC) isolated from patients with acute myeloid leukemia (AML), GPI-PLD activity in BMMNC isolated from 78 patients with AML and 15 normal persons was measured by using GPI-anchored placental alkaline phosphatase (PLAP) as a substrate and Triton X-114 phase partitioning. The GPI-PLD mRNA expression was measured by semi-quantitive reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the mRNA expression level and activity of GPI-PLD in BMMNC from de novo AML patients were 1.86 +/- 0.32 and 46.96 +/- 7.15% respectively; the mRNA expression level and activity of GPI-PLD in BMMNC from completely remission and refractory or relapsed patients were 1.26 +/- 0.29, 33.36 +/- 5.13%and 1.79 +/- 0.19, 44.31 +/- 7.22%, while those in BMMNC from normal controls were 1.27 +/- 0.23, 35.38 +/- 5.15% respectively. The mRNA expression level and activity of GPI-PLD in de novo and refractory or relapsed patients were obviously higher than those in normal controls with significant difference (p < 0.01), while the comparison between remitted patients and normal controls showed no statistical difference (p > 0.05). It is concluded that the expression level of GPI-PLD mRNA coincides with GPI-PLD activity. The mRNA expression and activity of GPI-PLD in de novo and refractory or relapsed patients are obviously higher than those in normal controls. It is worthy of further exploring whether GPI-PLD plays a certain role in process of leukemia pathogenesis.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Phospholipase D / RNA, Messenger / Bone Marrow Cells / Leukemia, Myeloid, Acute / Case-Control Studies / Cell Biology / Genetics / Metabolism Type of study: Observational study Limits: Adolescent / Adult / Child / Female / Humans / Male Language: Chinese Journal: Journal of Experimental Hematology Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Phospholipase D / RNA, Messenger / Bone Marrow Cells / Leukemia, Myeloid, Acute / Case-Control Studies / Cell Biology / Genetics / Metabolism Type of study: Observational study Limits: Adolescent / Adult / Child / Female / Humans / Male Language: Chinese Journal: Journal of Experimental Hematology Year: 2010 Type: Article