The effect of focal-adhesion micromanipulation in trauma and wound healing / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effect of focal-adhesion micromanipulation on the biological behavior of fibroblast.</p><p><b>METHODS</b>Micro-pot was made by microcontact printing. The molecules of constitutive protein was adhered on micro-pot by self-assemble of peptides. Skin fibroblasts were cultured on the membrane by self-made biomechanical cell culture for 2 weeks. Morphology observation and cell immunohistochemistry analysis was performed.</p><p><b>RESULTS</b>After 2 weeks, the morphology of the fibroblasts was diverse and more compliant. Cell immunohistochemistry analysis found that the expression of integrinbeta1, alpha5 and tensin was dramatically reduced.</p><p><b>CONCLUSIONS</b>The morphology and the biological behaviour of the fibroblasts in hypertrophic scar can be changed after micromanipulation of focal adhesion.</p>