Strategy for the identification of amplified genes in tumors / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 474-478, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-328846
ABSTRACT
Amplification of genomic DNA is often observed in tumors. The identification of genes in amplified regions may be helpful to the discovery of oncogenes associated with a specific tumor. Array-based comparative genomic hybridization and restriction landmark genomic scanning are applicable to the definition of such regions. The copy number alterations of target genes present in these regions and the levels of their mRNA and protein can be characterized by quantitative PCR and tissue microarray staining with immunohistochemistry. Transfection, RNA interference, cDNA microarray or reverse transcription-multiplex ligation-dependent probe amplification will facilitate demonstrating the role of such amplified genes in the pathogenesis of a specific tumor.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Gene Amplification
/
Reverse Transcriptase Polymerase Chain Reaction
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
RNA Interference
/
Genetics
/
Methods
/
Neoplasms
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2004
Type:
Article
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