Structural characterization of 5' flanking regulatory region of DNA repair gene Rad51 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 248-251, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-328907
ABSTRACT
<p><b>OBJECTIVE</b>To clarify the regulatory elements of Rad51 gene in its 5'flanking region.</p><p><b>METHODS</b>Various constructs were obtained by cloning different DNA fragments into pGL3 reporter vector. These constructs were then introduced into osteosarcoma cell line U2-OS by calcium phosphate method for transient expression of reporter gene, and luciferase activities were measured by luciferase assay.</p><p><b>RESULTS</b>Cells transfected with pGL3 constructs containing fragment -964 to +1430 and -733 to +1430 showed high luciferase activities. Obvious elevation of luciferase activities was also observed in cells transfected with pGL3 constructs containing four shorter derivative fragments -964 to -412, -746 to -412, -651 to -412 and -536 to -412. The highest luciferase activities were measured in transfected cells with plasmids containing fragment -964 to -412, and the lowest were in transfected cells with plasmids containing fragment -536 to -412. Luciferase activities in transfected cells with plasmids containing fragment -651 to -412 were higher than that in transfected cells with plasmids containing fragment -746 to -412.</p><p><b>CONCLUSION</b>It is believable that the basic transcription-promoting element (promoter) for Rad51 gene resides between -536 to -412, and two transcription-enhancing elements (enhancer) or binding sites of positive transcription factors reside between -651 to -536 and -964 to -746, whereas one transcription-inhibiting element (silencer) or binding site of negative transcription factor may reside between -746 to -651.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Promoter Regions, Genetic
/
5' Flanking Region
/
DNA-Binding Proteins
/
DNA Repair
/
Rad51 Recombinase
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2004
Type:
Article
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