Experimental study of plasmid TGF-beta 1 DNA gene transfer with lipofectamine into rabbit corneal epithelial cells in vitro / 华中科技大学学报（医学）（英德文版）
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 62-65, 2002.
Article
in English
| WPRIM
| ID: wpr-329129
ABSTRACT
To investigate whether the TGF-beta 1 plasmid DNA carried by lipofectamine could be introduced into cultured rabbit corneal epithelial cells, specific expression of the plasmid pMAM TGF-beta 1 in the cultured corneal epithelial cells was studied. Two days after 12 h of transfection of pMAMT-GF-beta 1 mediated by lipofectamine into the cultured corneal epithelial cells, the TGF-beta 1 protein expression specific for pMAMTGF-beta 1 in the cells was detected by means of immunohistochemical staining and the positive rate was 23.37%. The results suggested that foreign plasmid DNA could be effectively delivered into cultured rabbit corneal epithelial cells by means of lipofectamine, and this will provide a promising method of studying TGF-beta 1 on the mechanism of physiology and pathology concerned with corneal epithelial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plasmids
/
Cell Division
/
Cells, Cultured
/
Transforming Growth Factor beta
/
Gene Transfer Techniques
/
Epithelium, Corneal
/
Cell Biology
/
Transforming Growth Factor beta1
/
Genetic Vectors
Limits:
Animals
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2002
Type:
Article
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