An experimental study on the expression of heme oxygenase-2 mRNA in Hirschsprung's disease / 华中科技大学学报（医学）（英德文版）
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 152-154, 2002.
Article
in English
| WPRIM
| ID: wpr-329155
ABSTRACT
In order to investigate the relationship between the expression of heme oxygenase-2 (HO-2) mRNA and the pathogenesis of Hirschsprung's disease (HD), total ribonucleic acid (RNA) was extracted in the aganglionic and ganglionic segments of colon respectively from 15 cases of HD. The single-stranded cDNA of HO-2 was synthesized and further amplified by reverse transcription-polymerase chain reaction (RT-PCR). The expression of HO-2 mRNA was normal in ganglionic segments, but absent in aganglionic segments. It is concluded that the absence of HO-2 mRNA expression may be an important mechanism responsible for HD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Colon
/
Genetics
/
Heme Oxygenase (Decyclizing)
/
Hirschsprung Disease
Limits:
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
/
Infant, Newborn
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2002
Type:
Article
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