Study of rat osteoblasts transfected by transforming growth factor beta 1 gene / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 116-143, 2002.
Article
in English
| WPRIM
| ID: wpr-329167
ABSTRACT
In order to investigate the effect of TGF beta 1 gene transfer on the biological characteristics, the effects of gene transfer and supernatant of transfected osteoblasts on the proliferation and ALP activity of osteoblasts were detected by 3H-TdR and MTT. Our results showed that TGF beta 1 gene transfer had no effect on the biological characteristics and the activated supernatant of transfected osteoblasts stimulated proliferation and inhibited ALP activity of osteoblasts. TGF beta 1 gene transfer could promote the expression of TGF beta 1 and the biological characteristics of transfected osteoblasts were stable, which might be helpful for gene therapy of bone defects in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Recombinant Proteins
/
Transfection
/
Cell Division
/
Cells, Cultured
/
Transforming Growth Factor beta
/
Gene Transfer Techniques
/
DNA, Complementary
/
Cell Biology
/
Tissue Engineering
Limits:
Animals
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2002
Type:
Article
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