High glucose promotes vascular smooth muscle cell calcification by activating WNT signaling pathway / 南方医科大学学报
Journal of Southern Medical University
;
(12): 29-33, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-329179
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether high glucose-induced vascular calcification is associated with WNT signaling pathway.</p><p><b>METHODS</b>An in vitro model of human vascular smooth muscle cell (VSMC) calcification was induced by exposure of the cells to high glucose. The expressions of WNT signal molecules and bone-related proteins including Cbfa1, Osx, OCN and BMP2 were analyzed with qRT-PCR, and the cell calcification was assessed by alizarin red staining. The effect of Dkk1, a WNT signaling inhibitor, on high glucose-induced cell calcification was tested with alizarin red staining and calcium content analysis.</p><p><b>RESULTS</b>High glucose activated WNT signaling pathway in human VSMCs by up-regulating the expressions of WNT signal molecules including Wnt3a, Wnt7a, Fzd4 and Wisp1 mRNA by 1.86, 1.68, 2.1, and 2.3 folds, respectively, and by promoting the phosphorylation of β-catenin (2.70∓0.22, P<0.05), a key mediator of WNT signaling pathway. Inhibition of WNT signaling pathway by Dkk1 attenuated high glucose-induced VSMC calcification and down-regulated the expression of bone-related proteins Cbfa1, Osx, OCN, and BMP2 by (51∓9)%, (58∓11)%, (56∓10)%, and (62∓10)% (P<0.01).</p><p><b>CONCLUSION</b>WNT signaling pathway is involved in high glucose-induced VSMC calcification.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Up-Regulation
/
Cells, Cultured
/
Chemistry
/
Cell Biology
/
Myocytes, Smooth Muscle
/
Vascular Calcification
/
Wnt Signaling Pathway
/
Glucose
/
Muscle, Smooth, Vascular
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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