Role of PRKCD and ASK1 in U937 cell differentiation / 南方医科大学学报
Journal of Southern Medical University
;
(12): 17-22, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-329181
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of ASK1 and PRKCD in the process of monocyte differentiation, and explore their role in functional changes of hypersplenism spleen macrophages (Mφ) in portal hypertension (PH).</p><p><b>METHODS</b>U937 cells were stimulated to differentiate into monocyte/macrophage-like cells by cultivation in PMA and the mRNA expressions of ASK1 and PRKCD were detected by q-PCR and the changes of protein expression were identified by western blot analysis. The secretion of phagocytose related cytokines such as IL-10 and TNF-α were tested by ELISA, and the function of the macrophage-like cells were studied by chicken red blood cell phagocytose test.</p><p><b>RESULTS</b>The expressions of PRKCD and ASK1 mRNA were gradually decreased along with the cell differentiation, while the secretion of TNF-α was increased, IL-10 secretion reached a maximum at 24 h after PAM stimulation, and then gradually fell. The expression of ASK1 and p-ASK1 were rapidly increased compared with the non-stimulated U937 cells, while the expression of PRKCD and p-PRKCD were sightly declined. The phagocytose test show that U937 cells induced with PMA were able to swallow the chicken red blood cell.</p><p><b>CONCLUSION</b>Up-regulated protein expression of ASK1 and p-ASK1 and down-regulated protein expression of PRKCD and p-PRKCD in the process of PMA induced monocyte differentiation, are consist with the expression changes of splenic macrophage phagocytosis in hypersplenism, which leads to increased activity of Mφ.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phagocytosis
/
Physiology
/
RNA, Messenger
/
Down-Regulation
/
Cell Differentiation
/
Tumor Necrosis Factor-alpha
/
Interleukin-10
/
Bodily Secretions
/
U937 Cells
/
Cell Biology
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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