Expressions of Bim and ERKs in multidrug-resistant hepatic carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1838-1841, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-329188
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expressions of extracellular signal-regulated kinase (ERK) and the BH3-only subgroup of Bcl-2 related proteins (Bim) in multidrug-resistant hepatocellular carcinoma (HCC) cells and their association with drug resistance in the cells.</p><p><b>METHODS</b>The multdrug-resistant HepG-2 cell line was established by treatment with gradually increasing doses of ADM. CCK-8 assay was used to determine the drug sensitivity of the cells, and the expressions of MRP-1, P-gp, ERK1, ERK2, ERK5, and Bim were detected with Western blot. Bim mRNA expression level was measured using quantitative real-time PCR.</p><p><b>RESULTS</b>The drug resistance indices to ADM, 5-FU and CDDP was 6.8, 4.10, and 4.5 in HepG-2/ADM cells, respectively. The drug-resistantcells showed marked up-regulation of MRP-1, P-gp, ERK1, ERK2 and ERK5 with down-regulated phosphorylated ERK2 protein expression but no significant changes in phosphorylated ERK1 protein expression to result in a decreased ratio of P-ERK1/2 and P-ERK1/2. Bim mRNA and protein expressions were both decreased in HepG-2/ADM cells.</p><p><b>CONCLUSION</b>ERK and Bim are related to multidrug resistance in HepG-2/ADM cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Down-Regulation
/
Up-Regulation
/
Carcinoma, Hepatocellular
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Extracellular Signal-Regulated MAP Kinases
/
Hep G2 Cells
/
Liver Neoplasms
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2014
Type:
Article
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