Alternative polyadenylation site analysis of tumor-related genes based on 3'RACE in gastric cancer cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1738-1742, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-329210
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric cancer cells.</p><p><b>METHODS</b>We used 3'RACE to capture the APA sites of two tumor-related genes (HSP90α and SEC11A) in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database.</p><p><b>RESULTS</b>We found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3'UTRs.</p><p><b>CONCLUSIONS</b>There are new mRNA isoforms of HSP90α and SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stomach Neoplasms
/
Cell Transformation, Neoplastic
/
Polyadenylation
/
Cell Line, Tumor
/
Genes, Neoplasm
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2014
Type:
Article
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