Live cell fluorescent imaging and cytotoxicity assessment of pH fluorescent probe based on styrylcyanine dyes / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1642-1645, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-329231
ABSTRACT
<p><b>OBJECTIVE</b>To prepare a pH fluorescence probe based on styrylcyanine dyes for live cell imaging.</p><p><b>METHODS</b>The Probe 1 was prepared by reaction of 4-pyridinecarboxaldehyde with 1,1,2-trimethylbenz[e]indole. The influence of pH on the fluorescent properties was examined, and the cell viability was examined using cell counting kit-8. The Probe 1 was used as a pH fluorescence probe in living cell.</p><p><b>RESULTS</b>Probe 1 emitted green fluorescence under neutral and basic conditions but orange fluorescence under acid condition. Probe 1 selectively stained the cytoplasmic regions of living cells without significantly affecting the cell viability.</p><p><b>CONCLUSION</b>The pH-sensitive fluorescent probe prepared based on styrylcyanine possesses good ability of cell membrane permeation for live cell fluorescent imaging.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cells, Cultured
/
Optical Imaging
/
Fluorescence
/
Fluorescent Dyes
/
Hydrogen-Ion Concentration
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2014
Type:
Article
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