Changes of circulating hematopoietic progenitor cells in patients undergoing coronary artery bypass graft surgery / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the changes of circulating hematopoietic progenitor cells (cHPCs) in patients undergoing coronary artery bypass graft (CABG) surgery.</p><p><b>METHODS</b>Fifty-one patients scheduled to undergo elective CABG surgery were prospectively enrolled in this study. Blood samples were collected at baseline and at 1, 3, and 5 days after the surgery. CD34⁺ CD45dim, CD133⁺ CD45dim, and CD34⁺ CD133⁺ CD45dim cells were measured by flow cytometry. The functionality of the circulating progenitor cells were studied in vitro using clonogenic and migration assays.</p><p><b>RESULTS</b>All the elective patients showed a significant postoperative increase of CD34⁺ CD45dim (1.51 ± 1.26 vs 2.02 ± 1.16, P=0.036) and CD133⁺ CD45dim cells (1.16 ± 0.85 vs 1.65 ± 0.99, P=0.008) irrespective of on-pump or off-pump CABG. The perioperative changes of the cHPCs was more significant in patients undergoing on-pump surgery than those having off-pump surgery.</p><p><b>CONCLUSION</b>In patients undergoing elective cardiac surgery, cHPC level increased early after the surgery but recovers the normal level over time.</p>