Study on the relationship of MTHFR polymorphisms with unexplained recurrent spontaneous abortion / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 39-42, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-329403
ABSTRACT
<p><b>OBJECTIVE</b>To assess the relationship of methylenetetrahydrofolate reductase (MTHFR) C677T genotypes to unexplained recurrent spontaneous abortion (URSA).</p><p><b>METHODS</b>This study included two groups57 currently non-pregnant women with a history of URSA (URSA group), and 50 currently non-pregnant women with a history of having given birth to at least one live baby and without any history of spontaneous abortion, still-born fetus, placental thrombosis and intrauterine growth retardation(IUGR)(control group). The fasting serum-Hcy was measured with high pressure liquid chromatography. Folic acid and vitamin B(12) were detected by radioimmune assay; antiphospholipid antibody (ACA) was detected by ELISA. MTHFR C677T gene polymorphisms were detected by the technique of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).</p><p><b>RESULTS</b>C/C genotype in URSA group was significantly lower than that in control group, the total mutant T allele frequency was significantly higher than that in control group. There was no significant difference in respect of "age, rural area/city, period, primary/secondary abortion" between the genotype distributions of MTHFR C677T. The T/T genotype and C/T+T/T genotypes frequencies for "abortion times>or=3" were higher than those for "abortion time <3".</p><p><b>CONCLUSION</b>MTHFR C677T gene polymorphism is a genetic risk factor for URSA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
Vitamin B 12
/
Blood
/
Polymorphism, Restriction Fragment Length
/
DNA Mutational Analysis
/
China
/
Abortion, Habitual
/
Polymerase Chain Reaction
/
Point Mutation
/
Methylenetetrahydrofolate Reductase (NADPH2)
Limits:
Adult
/
Female
/
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2004
Type:
Article
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