Effects of AGEs on oxidation stress and antioxidation abilities in cultured astrocytes / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 79-86, 2004.
Article
in English
| WPRIM
| ID: wpr-329653
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether two kinds of in vitro prepared advanced glycation end products (AGEs), Glu-BSA and Gal-BSA, could change oxidation stress and anti-oxidation abilities in astrocytes, and thus might contribute to brain injury.</p><p><b>METHODS</b>Changes of GSH, MDA, SOD, MAO-B, nitric oxide were measured after AGEs treatment.</p><p><b>RESULTS</b>Both 0.1 g/L Glu-BSA and Gal-BSA could slightly decrease GSH level, while 1 g/L of them significantly decreased GSH level by 35% and 43% respectively. The MDA levels of both 1 g/L AGEs treated groups (306 +/- 13 and 346 +/- 22) were higher than that of the normal group (189 +/- 18), which could be inhibited by free radical scavenger NAC. The SOD activities of both 1 g/L AGEs treated groups (67.0 +/- 5.2 and 74.0 +/- 11.0) were lower than that of the normal group (85.2 +/- 8.0). Both 0.1 g/L AGEs could slightly increase the activity of MAO-B, while 1 g/L of them could increase MAO-B activity by 1.5 and 1.7 folds respectively. Both AGEs stimulation could produce NO level by 1.7 and 2 folds respectively.</p><p><b>CONCLUSION</b>Enhanced levels of astrocytic oxidation stress and decrease of antioxidation abilities may contribute to, at least partially, the detrimental effects of AGEs in neuronal disorders and aging brain.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Superoxide Dismutase
/
Serum Albumin, Bovine
/
Cells, Cultured
/
Cerebral Cortex
/
Astrocytes
/
Rats, Wistar
/
Glycation End Products, Advanced
/
Oxidative Stress
/
Cell Biology
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2004
Type:
Article
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