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Differentiation of rat neural stem cells and its relationship with environment / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 1-7, 2004.
Article in English | WPRIM | ID: wpr-329661
ABSTRACT
<p><b>OBJECTIVE</b>To explore the differentiation fates of rat neural stem cells (NSCs) in different environmental conditions.</p><p><b>METHODS</b>NSCs derived from 16-day-old rat embryo were proliferated in vitro and implanted into the brain of rats with intra-cerebral hemorrhage. At the same time some NSCs were co-cultured in vitro with Schwann cells derived from newborn rats. MAP-2, GFAP and GalC (which are the specific markers of neural cells, astrocytes and oligodendrocytes respectively), BrdU and beta-tubulin were detected by immunohistochemical and immunofluorescent methods.</p><p><b>RESULTS</b>BrdU positive cells that were implanted into the brain distributed around the hemorrhagic area. The majority of them were GFAP positive astrocytes while a few of them were beta-tubulin positive neural cells or GalC positive oligodendrocytes. After being co-cultured with Schwann cells in vitro, NSCs are predominately shown beta-tubulin and MAP-2 positive, and only a minority of them were GFAP or GalC positive.</p><p><b>CONCLUSIONS</b>The hemorrhagic environment in vivo induces NSCs to differentiate mainly into astrocytes while co-culture with Schwann cells in vitro induce the majority of NSCs to differentiate into neural cells.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Physiology / Schwann Cells / Sciatic Nerve / Stem Cells / General Surgery / Caudate Nucleus / Cell Differentiation / Cell Movement / Cells, Cultured Limits: Animals Language: English Journal: Biomedical and Environmental Sciences Year: 2004 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Physiology / Schwann Cells / Sciatic Nerve / Stem Cells / General Surgery / Caudate Nucleus / Cell Differentiation / Cell Movement / Cells, Cultured Limits: Animals Language: English Journal: Biomedical and Environmental Sciences Year: 2004 Type: Article