Construction and expression of the recombinant plasmid containing BddhFVIII in HepG2 cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 259-262, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-329893
ABSTRACT
<p><b>OBJECTIVE</b>To get stable cell line expressing B domain-deleted human FVIII (BDDhFVIII) by constructing the eukaryotic expression plasmid.</p><p><b>METHODS</b>Eukaryotic expression plasmid containing BDDhFVIII was constructed and transfected into HepG2 cells via electroporation. The expression and purification of the target protein was detected by Western blot.</p><p><b>RESULTS</b>Results of enzyme digestion and sequence analysis demonstrated that the gene of BDDhFVIII was correctly inserted into the eukaryotic expression vector pcDNA4/v5-his. Western blot confirmed the successful expression of BDDhFVIII at the protein levels in HepG2 cells.</p><p><b>CONCLUSION</b>The constructed eukaryotic expression vector was able to generate high level expression of human FVIII in HepG2 cells, thus could construct human blood coagulation FVIII stable cell line successfully.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Recombination, Genetic
/
Factor VIII
/
Gene Expression
/
Electroporation
/
Hep G2 Cells
/
Genetic Vectors
/
Genetics
/
Hemophilia A
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2012
Type:
Article
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