Application of recording SK2 current in human atrial myocytes by perforated patch clamp techniques with the mix of beta-escin and amphotericin B / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 214-218, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-329905
ABSTRACT
<p><b>OBJECTIVE</b>To establish a perforated patch-clamp technology with amphotericin B and beta-escin and to research the regulation of small conductance calcium-activated potassium channel SK2 currents by calcium ions.</p><p><b>METHODS</b>Single human atrial myocytes were enzymatically isolated from the right atrial appendage. Amphotericin B and / or beta-escin were used by perforated electrode liquid. The regulation of SK2 current by calcium ions in human atrial myocytes was performed with the perforated patch-clamp technique. The intracellular calcium changes were measured by the intracellular calcium test system.</p><p><b>RESULTS</b>Mixed perforated electrode liquid compared with 150 microg/ml amphotericin B or 6.88 microg/ml beta-escin alone, it was easy to seal cells and activate SK2 current by the former method. Moreover, the ration of F340/380 was consistent with the change of intracellular free calcium ion concentration increase after the formation of perforation. The ration of F340/380 was measured by intracellular calcium test system.</p><p><b>CONCLUSION</b>The appropriate concentration of amphotericin B mixed with beta-escin can form a stable whole-cell patch recording technology that is appropriate for the research of SK2 current regulation by intracellular calcium.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Amphotericin B
/
Calcium
/
Patch-Clamp Techniques
/
Potassium Channels, Calcium-Activated
/
Myocytes, Cardiac
/
Electric Conductivity
/
Escin
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2012
Type:
Article
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