Nicotine regulates large conductance ca2+ activated K+ channels in rat coronary arterial smooth muscle cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 24-27, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-329955
ABSTRACT
<p><b>OBJECTIVE</b>The present study was to explore signaling mechanisms underlying nicotine-induced inhibition of large-conductance calcium-activated potassium channels (BK(Ca)).</p><p><b>METHODS</b>8 week male Wistar rats were divided randomly into saline group and nicotine group and received respectively injection with saline or nicotine (Sigma, Shanghai, China) at 2 mg/(kg x d) for 21 days. Coronary vascular smooth muscle cells were dissociated enzymatically. Dissociated smooth muscle cells were interfered with CPT-cAMP (100 micromol/L) or forskolin (10 micromol/L). The signal channel open dwell-time (To), close dwell-time (Tc) and open probability (Po) were recorded.</p><p><b>RESULTS</b>CPT-cAMP or forskolin significantly prolonged To, shorten Tc and increased Po in saline group (P < 0.01). But in nicotine group To, Tc and Po did not been changed.</p><p><b>CONCLUSION</b>This phenomenon may serve as a physiological mechanism that nicotine inhibits BK(Ca) channel activity to increase via cAMP/PKA-dependent pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Arteries
/
Signal Transduction
/
Rats, Wistar
/
Patch-Clamp Techniques
/
Coronary Vessels
/
Cell Biology
/
Myocytes, Smooth Muscle
/
Large-Conductance Calcium-Activated Potassium Channels
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS