A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 510-512, 2011.
Article
in Zh
| WPRIM
| ID: wpr-329963
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method of acutely isolating dorsal root ganglion (DRG) neurons for patch clamp study of single-channel.</p><p><b>METHODS</b>DRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing.</p><p><b>RESULTS</b>The acutely isolated DRG cells were easy to form the higher sealing resistance (> 5G Omega), which lowered noise level, so that pA-level single channel currents could be recorded.</p><p><b>CONCLUSION</b>The acutely isolated DRG neurons in this study are an ideal for patch-clamp study of single-channel.</p>
Full text:
1
Index:
WPRIM
Main subject:
Physiology
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Ion Channel Gating
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Cell Separation
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Rats, Sprague-Dawley
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Patch-Clamp Techniques
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Cell Biology
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Ganglia, Spinal
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Ion Channels
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Methods
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Neurons
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Applied Physiology
Year:
2011
Type:
Article