The study of cell biocompatibility of new pattern biphasic calcium phosphate nanocomposite in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 106-109, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-329975
ABSTRACT
<p><b>OBJECTIVE</b>To study the cell biocompatibility of porous biphasic calcium phosphate nanocomposite in vitro.</p><p><b>METHODS</b>Bone marrow mesenchymal cell (BMSCs) obtained from SD rat bone marrow were in vitro induced and proliferated. Afler their osteoblast phenotypes were verified, BMSCs were seeded onto prepared porous biphasic calcium phosphate nanocomposite (Experiment group) and common porous hydroxyapatite (Control group). The cell adhesion was evaluated by scanning electron microscope. Synthesis of alkaline phosphatase enzyme (ALP) and osteocalcin were detected and cell cycle was detected by flow cytometry.</p><p><b>RESULTS</b>BMSCs could fully attach to and extend on the material in experiment and control group, Moreover, experiment group were superior to control group in adhesion, proliferative abilities and osteogenic activity.</p><p><b>CONCLUSION</b>BMSCs can differentiate to osteoblast phenotype; the porous biphasic calcium phosphate nanocomposite as bone tissue engineering scaffold has good cell biocompatibility.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Bone and Bones
/
Materials Testing
/
Bone Marrow Cells
/
Osteocalcin
/
Cell Adhesion
/
Durapatite
/
Tissue Engineering
/
Alkaline Phosphatase
/
Nanocomposites
Limits:
Animals
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2005
Type:
Article
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