The positive effect of transforming growth factor beta on ectomesenchymal stem cells of embryonic facial processes differentiating to smooth muscle cells / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 460-462, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-330021
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of transforming growth factor beta (TGF-beta) on ectomesenchymal stem cells differentiating to smooth muscle cells.</p><p><b>METHODS</b>60 pmol/L TGF-beta was added to the ectomesenchymal stem cells of embryonic facial processes. Immunohistochemistry assay and image analysis were used to value the expression extent of a smooth muscle actin (alpha-SMA) and quantitative RT-PCR was used to value the quantity of alpha-SMA.</p><p><b>RESULTS</b>2 days later, about 95% cells in TGF-beta group and 65% cells in control group without differentiation inhibitor expressed alpha-SMA. Expression of alpha-SMA in TGF-beta group was stronger than that of control group after one and two days. Quantitative RT-PCR showed the quantity of alpha-SMA mRNA in treated group cells was more than that of in control group.</p><p><b>CONCLUSION</b>Quantity of alpha-SMA in TGF-beta group is more than that of spontaneous differentiation group. TGF-beta has positive effect on ectomesenchymal stem cells differentiating to smooth muscle cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cell Differentiation
/
Cells, Cultured
/
Transforming Growth Factor beta
/
Actins
/
Cell Biology
/
Myocytes, Smooth Muscle
/
Mesenchymal Stem Cells
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2004
Type:
Article
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