Three subtypes expressions of leptin receptor in the rat anterior pituitary and influence of leptin on intracellular free Ca2+ of the rat growth hormone cell / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 349-353, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-330098
ABSTRACT
<p><b>AIM</b>To observe the expression of Leptin receptors (OB-R) in male rat anterior pituitary, and study the influence of Leptin on the level of intracellular free Ca2+ ([Ca2+]i) in the cultured growth hormone (GH) cell of male rat pituitary.</p><p><b>METHODS</b>RT-PCR method was used to observe the expression of Leptin receptors (OB-R) in male rat anterior pituitary. We used grade centrifuging method to get growth hormone (GH) cell, and [Ca2+]i in GH cell was examined by laser scanning confocal system.</p><p><b>RESULTS</b>OB-R mRNA were expressed in male rat anterior pituitary, including OB-R (common form), OB-Ra (short form) and OB-Rb (long form). There were about 70% or 80% GH cell by grade centrifuging. Leptin at 10(-8)mol/L could decrease the level intracellular free Ca2+ ([Ca2+]i) in cultured GH cell.</p><p><b>CONCLUSION</b>There are three subtypes of Leptin receptors expressions in male rat anterior pituitary, and Leptin could reduce intracellular free Ca2+ level of GH cell markedly.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pituitary Gland
/
Growth Hormone
/
Cells, Cultured
/
Calcium
/
Rats, Sprague-Dawley
/
Receptors, Cell Surface
/
Receptors, Leptin
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2004
Type:
Article
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