Expression of ryanodine receptor isoforms in airway smooth muscle cells of rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 181-184, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-330151
ABSTRACT
<p><b>AIM</b>To detect the expression of ryanodine receptor (RyR) subtypes in normol rat airway smooth muscle cells(ASMCs) and changes during chronic asthma formation.</p><p><b>METHODS</b>ASMCs were cultured with collagen enzyme digestion method. The expression of subtypes of RyR were detected by RT-PCR. Purified PCR product linked with pGEM-T vector to make DNA sequence assay. Chronic asthma model was made with OVA, the changes of RyRs detected by RT-PCR.</p><p><b>RESULTS</b>All subtypes of RyR were expressed in airway smooth muscle cells of normol rat. The expression of RyR1 increased obviously compared with control group (P < 0.05) on chronic asthma.</p><p><b>CONCLUSION</b>Co-expression of three subtypes of RyR in ASMCs of normal rat, indicate that there are complicated intercellular Ca2+ regulation mechanism in ASM, moreover RyR1 might play a role during asthma development.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asthma
/
Bronchi
/
RNA, Messenger
/
Rats, Wistar
/
Ryanodine Receptor Calcium Release Channel
/
Protein Isoforms
/
Myocytes, Smooth Muscle
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2004
Type:
Article
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