Protective effects of adenovirus-mediated hepatocyte growth factor on injury of rat cortex neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 156-160, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-330158
ABSTRACT
<p><b>AIM</b>To investigate the protective effects of adenovirus-mediated hepatocyte growth factor (Ad-HGF) on injury of rat cortex neurons induced by in vitro serum-free culture.</p><p><b>METHODS</b>Flow cytometry was used to assay the transfection rate of rat cortex neurons infected by adenovirus-mediated green fluorescent protein(Ad-GFP) at different multiplicity of infection (MOI) to find out the best MOI in experiment. ELISA was used to elucidate the expression patterns of cortex neuron. Neutral red stain and PI-Hoechst 33342 double stain were used to compare the viability of cortex neurons, which were cultured in serum-free medium for 6 h, 12 h, 24 h and 48 h respectively, among the Ad-HGF transfected group, the Ad-GFP transfected group and the control group.</p><p><b>RESULTS</b>It was found that when MOI was 50 PFU per cell, a transfection rate as high as 99.3% was maintained and Ad-HGF was able to express in cortex neurons effectively and persistently. In addition, the death rate and apoptotic rate of cortex neurons (infected 2 hours after seeding) cultured in serum-free medium for 12 h in Ad-HGF transfected group was significantly lower than that in both the Ad-GFP group and the control group (P < 0.05).</p><p><b>CONCLUSION</b>Ad-HGF plays a protective role against in vitro serum-free culture induced injury on rat cortex neurons infected 2 hours after seeding. Though its effects on rat cortex neurons infected 5 days after seeding are not so remarkable, Ad-HGF also has the potential to protect cortex neurons from serum-free culture induced injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Transfection
/
Cells, Cultured
/
Cerebral Cortex
/
Adenoviridae
/
Culture Media, Serum-Free
/
Rats, Wistar
/
Hepatocyte Growth Factor
/
Genetics
/
Animals, Newborn
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2004
Type:
Article
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