Molecular identification of hairy antler by analysis of high resolution melting / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 619-623, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-330189
ABSTRACT
High resolution melting (HRM) , an important technology for genotyping and mutation scanning, has broad prospects in the authenticity of traditional Chinese medicine. This paper selected universal CO I primers and used HRM to establish a new method for authenticity of Hairy Antler. PCR was conducted at the annealing temperature of 60 °C and 45 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further optimized. The results showed that the Tm values of Cervus nippon were (81.96 ± 0.07), (84.51 ± 0.03) °C and Cervus elaphus was(82.58 ± 0.13), (85.95 ± 0.05) °C with 10-100 mg · L(-1) DNA template, 0.2 µLmol · L(-1) primer, 2.0 mmol · L(-1) Mg2+. This method can authenticate of hairy antler and is simple, fast, high-throughput, visualization.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antlers
/
Reference Standards
/
DNA
/
Deer
/
Chemistry
/
Polymerase Chain Reaction
/
Classification
/
DNA Primers
/
Transition Temperature
/
Genetics
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article
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