Effect of shikonin on proliferation of keratinocytes induced by interleukin-17 and expression of chemokines / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 946-949, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-330205
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of shikonin on the proliferation of human keratinocytes induced by IL-17 and secretion of chemokines, in order to discuss the mechanism of Shikonin in the treatment of psoriasis.</p><p><b>METHOD</b>In vitro cultured HaCaT cells were stimulated by IL-17A (200 μg x L(-1)) and mixed with different concentrations (2, 1 mg x L(-1)) of shikonin for 24 hours. The cell proliferation was detected by CCK-8 assay. Cell secretion inflammatory factor interleukin-23 (IL-23) was detected by ELISA. The expressions of intracellular chemokines CXCL1, CXCL2, CCL20 and 6-defensin 4 (DEFB4) were detected by Real-time PCR.</p><p><b>RESULT</b>Shikonin (2,1 mg x L(-1)) could distinctly inhibit HaCaT cell proliferation induced by IL-17A, with statistical difference (P < 0.01). Each shikonin group showed decreases in the secretion of IL-23 and inhibition in expressions of intracellular CXCL1, CXCL2, CCL20 and DEFB4.</p><p><b>CONCLUSION</b>Shikonin could inhibit HaCaT cells proliferation induced by IL-17 and secretion of relevant cytokines and recruit leukocytes by inhibiting chemokines, so as to show the effect in treating psoriasis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Drugs, Chinese Herbal
/
Keratinocytes
/
Cell Line
/
Naphthoquinones
/
Chemokines
/
Interleukin-17
/
Cell Biology
/
Cell Proliferation
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article
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