A cell membrane chromatography method for screening 5-HT receptor agonists from drug pair of Chuanxiong Rhizoma and Angelicae Dahuricae Radix / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 490-494, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-330249
ABSTRACT
Migraine is one of the common and frequently encountered diseases. The study proves that 5-hydroxytryptamine (5-HT) receptor, plays an important role in the occurrence of migraine. Rat striatum was used for preparation of the cell membrane stationary phase (CMSP) in our experiments. The cell membrane chromatography (CMC)-offline-HPLC system was applied to specifically recognize the components from the drug pair of Chuanxiong Rhizoma and Angelicae Dahuricae Radix, which interact with the receptors on CMSP. The dissociation equilibrium constant (KD) was measured in a rat striatum/CMC system, performed by continuously pumping sumatriptan, a 5-HT1D agonist, ranging from 2.42 x 10(-8) to 4.84 x 10(-7) mol · L(-1) through a CMC column, and the capacity factors (k') were recorded. The KD value obtained from the model was (4.59 ± 0.33) x 10(-6) mol · L(-1) for imperatorin, and the rat model of migraine induced by nitroglycerin was applied to validate the pharmacological effects of the drug pair. The results indicated that the CMC method could be a quick and efficient way for characterizing the drug-receptor interactions in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Cell Membrane
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Serotonin Receptor Agonists
/
Rats, Sprague-Dawley
/
Angelica
/
Receptor, Serotonin, 5-HT1D
/
Drug Evaluation, Preclinical
/
Drug Therapy
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS