Identification of pyrrosiae folium and its adulterants based on psbA-trnH sequence / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2222-2226, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-330316
ABSTRACT
In this study, the psbA-trnH sequence as DNA barcode was used to evaluate the accuracy and stability for identification pteridophyte medicinal material Pyrrosiae Foliumas from adulterants. Genomic DNA from 106 samples were extracted successfully. The Kimura 2-Parameter (K2P) distances and ML tree were calculated using software MEGA 6.0. The intra-specific genetic distances of 3 original plants were lower than inter-specific genetic distances of adulterants. The ML tree indicated that Pyrrosiae Folium can be distinguished from its adulterants obviously. Therefore, the psbA-trnH sequence as a barcode of the pteridophyte, can accurately and stably distinguish Pyrrosiae Folium from its adulterants.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Plant Proteins
/
Quality Control
/
Drugs, Chinese Herbal
/
Molecular Sequence Data
/
Base Sequence
/
Chemistry
/
Drug Contamination
/
Classification
/
DNA, Ribosomal Spacer
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article
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