Identification of moutan cortex and its adulterants by ITS2 sequence / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2180-2183, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-330325
ABSTRACT
To explore a new method to identify Moutan Cortex to guarantee its safe use, internal transcribed spacer 2 (ITS2) sequence was used to identify Moutan Cortex and its adulterants. DNA was extracted and target fragments were amplified. Sequences were analyzed and assembled by CodonCode Aligner V3.7.1. Genetic distances were computed and phylogenetic tree was constructed based on kimura 2-parameter (K2P) model by MEGA 5.0. The length of the 20 ITS2 sequences of Moutan Cortex from nine different places is 227 bp, and no variation site was detected. The maximum inter-specificK2P distance of Moutan Cortex is 0, the minimum intra-specific K2P distance is 0.041, the average intra-specific K2P distance is 0.222. According to NJ analysis, Moutan Cortex from different places can get together as one branch with bootstrap support values 99%, which indicates Moutan Cortex can be easily distinguished from its adulterants. Using ITS2 sequence can accurately identify Moutan Cortex and its adulterants, it is an effective supplementary to traditional identification methods.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Quality Control
/
Drugs, Chinese Herbal
/
Molecular Sequence Data
/
Base Sequence
/
Chemistry
/
China
/
Drug Contamination
/
Classification
/
DNA, Plant
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article
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