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Identification of Bupleurum chinense and B. longiradiatum based on ITS2 barcode / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2160-2163, 2014.
Article in Chinese | WPRIM | ID: wpr-330329
ABSTRACT
In this study, ITS2 barcode was used to identify Bupleurum chinense and B. longiradiatum. The ITS2 regions of 48 samples were amplified and sequenced. The sequences obtained above were aligned and the K2P distances were calculated. We used three methods, BLAST1, nearest distance and phylogenetic tree (NJ-tree), to test the identification ability. The results showed that the maximum intraspecific genetic distance of B. chinense was 0.013, and the minimum interspecific genetic distance between B. chinense and B. longiradiatum was 0.049. The NJ-tree can easily identify B. chinense and B. longiradiatum. Therefore, the ITS2 barcode is suitable to identify B. chinense and B. longiradiatum.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Quality Control / Drugs, Chinese Herbal / Molecular Sequence Data / Chemistry / Classification / DNA, Plant / DNA, Ribosomal Spacer / Bupleurum / DNA Barcoding, Taxonomic Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Quality Control / Drugs, Chinese Herbal / Molecular Sequence Data / Chemistry / Classification / DNA, Plant / DNA, Ribosomal Spacer / Bupleurum / DNA Barcoding, Taxonomic Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2014 Type: Article