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Wnt/b-catenin signaling pathway affects the protein expressions of caspase-3, XIAP and Grp-78 in hepatocellular carcinoma / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 599-602, 2011.
Article in Chinese | WPRIM | ID: wpr-330686
ABSTRACT
To investigate the relationship and significance of Wnt/b-catenin signaling pathway with caspase-3, XIAP, HSP27and Grp-78. The HCC cell line HepG2 was transfected with small interfering RNA (siRNA) directed against b-catenin. After 72 and 96 h, protein was extracted and the protein expressions of b-catenin, caspase-3, XIAP, Grp-78 and HSP27 were detected by Western blot. b-catenin protein expression was inhibited at both time points and the expression at 96 h was higher than that at 72 h (F = 160.72, P is less than to 0.01). Interestingly, Caspase-3 protein expression was decreased at 72 h and increased to normal at 96 h (F = 136.10, P is less than to 0.01), while p-caspase-3 protein expression increased at 72 h and decreased to normal at 96 h (F = 98.65, P is less than to 0.01). XIAP protein expression decreased at 72 h (F = 37.29, P is less than to 0.01) and increased at 96 h. Grp-78 protein expression increased at 72 h and decreased to normal at 96 h ( F = 58.72, P is less than to 0.01). HSP27 protein expression showed no change following transfection ( F = 1.91, P is more than to 0.05). Wnt/b-catenin signaling pathway is related to the protein expressions of caspase-3, XIAP and Grp-78, but not related to HSP27 protein expression in HCC. Wnt/b-catenin signaling pathway may participate in the regulation of HCC apoptosis, proliferation and differentiation through affecting these factors.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Carcinoma, Hepatocellular / Beta Catenin / Catenins / Caspase 3 / Wnt Signaling Pathway / Liver Neoplasms / Metabolism Limits: Humans Language: Chinese Journal: Chinese Journal of Hepatology Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Carcinoma, Hepatocellular / Beta Catenin / Catenins / Caspase 3 / Wnt Signaling Pathway / Liver Neoplasms / Metabolism Limits: Humans Language: Chinese Journal: Chinese Journal of Hepatology Year: 2011 Type: Article