Construction and identification of blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1939-1942, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-330798
ABSTRACT
<p><b>OBJECTIVE</b>To construct and identify blood type B antigen mimetic polypeptide-macrophage inflammatory protein 3beta (Mip3beta) double expression recombinant plasmid.</p><p><b>METHODS</b>The positive phage clone P1 was obtained using phage random 12-mer peptide library. Specific primers were designed to amplify the phage DNA of P1 and transmembrane domain and inner segment of PBluscript-Fas gene. The products of the amplification were linked into Mip3betav21 to construct blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid. The recombinant plasmid was transfected into human melanoma cell line B16 to identify its expression.</p><p><b>RESULTS AND CONCLUSION</b>Blood type B antigen mimetic polypeptide-Mip3beta double expression recombinant plasmid is successfully obtained and expressed in human melanoma cell line B16.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Plasmids
/
Blood Group Antigens
/
Recombinant Fusion Proteins
/
Recombinant Proteins
/
Gene Expression
/
Cell Line, Tumor
/
Genetics
/
Macrophages
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
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