Construction of recombinant adenovirus containing TK gene and its effect against human liver cancer cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1887-1889, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-330813
ABSTRACT
<p><b>OBJECTIVE</b>To construct a replication-defective adenovirus containing TK gene and investigate the killing effects of TK gene against human liver cancer cells SMMC-7721.</p><p><b>METHODS</b>The recombinant adenovirus ADV-TK was constructed using homologous recombination in the cells. SMMC-7721 cells transfected with recombined adenovirus were exposed to GCV, and the cell viability was measured by MTT assays.</p><p><b>RESULTS</b>The recombinant adenovirus containing TK gene was successfully constructed. Transfection by the recombinant adenovirus ADV-TK and GCV exposure significantly suppressed the growth of SMMC-7721 cells.</p><p><b>CONCLUSION</b>A replication-defective adenovirus containing TK gene has been successfully constructed, and in combination with GCV, the recombinant adenovirus produces significant killing effect against SMMC-7721 cells in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Thymidine Kinase
/
Genetic Therapy
/
Adenoviridae
/
Cell Line, Tumor
/
Genetic Vectors
/
Genetics
/
Liver Neoplasms
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
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