Suramin inhibits the in vitro expression of encephalitis B virus proteins NS3 and E / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 375-379, 2003.
Article
in En
| WPRIM
| ID: wpr-330901
Responsible library:
WPRO
ABSTRACT
In this study, the mechanism by which Suramin inhibits the replication of epidemic encephalitis B virus was explored to provide a theoretical basis for its further application in clinical practice. After viral infection of HepG2 and IMR-32 cells, different concentrations of Suramin were added to the culture media, and then the cultural supernatants and infected cells were collected 48 h later. For the evaluation of the curative effect, cytopathic effect (CPE), virus titers, the expression of viral protein and viral RNA were determined by Western blot, RT-PCR and in vitro RNA synthesis, respectively. At the concentration of 50 microg/ml of Suramin, HepG2 and IMR-32 infected with epidemic encephalitis B virus decreased by 51.8% and 0.03% respectively, as compared with controls. It was suggested that expression of encephalitis B virus proteins NS3 and E was notably reduced by Suramin. This is especially true of E protein. At RNA level, however, no difference in RNA virus was found between Suramin-treated virus and non-treated cells. Our results suggest that Suramin can inhibit viral replication by blocking the production of viral proteins.
Full text:
1
Index:
WPRIM
Main subject:
Antiviral Agents
/
Pathology
/
Pharmacology
/
Suramin
/
Virology
/
Virus Replication
/
RNA, Viral
/
Serine Endopeptidases
/
Viral Envelope Proteins
/
Viral Nonstructural Proteins
Limits:
Humans
Language:
En
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2003
Type:
Article