Construction of antisense RNA expression plasmid for u-PAR and its transfection to highly invasive PC-3M cell subclones / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 369-372, 2003.
Article
in English
| WPRIM
| ID: wpr-330903
ABSTRACT
To evaluate the specific inhibition of antisense u-PAR on the u-PAR expressions in highly invasive cell subclones and to determine its blocking function in the invasion by those cells, a cDNA fragment of u-PAR obtained by RT-PCR was inserted into a plasmid vector named pcDNA3 in antisense orientation. Then the antisense u-PAR recombinant was transfected into highly invasive cell subclones. The u-PAR expression in neo-resistant cells was examined by RT-PCR and immunohistochemical assay. Compared to the control cells, the content of mRNA and protein of u-PAR in transfected cells decreased sharply, and the rate of inhibition was 53% and 73%, respectively, indicating that an antisense u-PAR might have played a specific inhibitory role in its expression in the cells, which may provide a good cell model for making further investigation of the inhibitory effects of the antisense u-PAR on invasion in highly invasive cell subclones of human prostate carcinoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Plasmids
/
Prostatic Neoplasms
/
Transfection
/
Urokinase-Type Plasminogen Activator
/
RNA, Antisense
/
Cloning, Molecular
/
Receptors, Cell Surface
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Line, Tumor
Limits:
Humans
/
Male
Language:
English
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2003
Type:
Article
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