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Selective depletion of the allo-antigen specific T cells by Fas/FasL pathway by cytokine IFN-gamma and IL-2 / 华中科技大学学报(医学)(英德文版)
Article in English | WPRIM | ID: wpr-330910
Responsible library: WPRO
ABSTRACT
To investigate the value of apoptosis of the allo-antigen specific T cells induced by Fas/FasL pathway in preventing graft-versus-host disease (GVHD), the CD34+ cells transfected with FasL or not, used as stimulus cells, were mixed with allo-antigen specific T lymphocytes in presence or absence of IFN-gamma and IL-2. After 5 days, apoptosis of T cells was detected by TdT nick end mediated dUTP labeling (TUNEL) and flow cytometry (FCM). The affects of these two cytokines on CD34+ cells in the graft were also compared. The ratio of apoptosis of T cells was 12.1+/-1.5% when CD34+ cells transfected with FasL was used as stimulus cells, much higher than that of CD34+ cells non-transfected (3.2+/-1.1%, P<0.01). And in presence of IFN-gamma or IL-2, the ratio reached 20.1+/-2.3%, 17.6+/-1.3% respectively (P<0.01). However, IFN-gamma up-regulated Fas expression of CD34+ cells and increased the sensibility of CD34+ cells to soluble FasL (sFasL); IL-2 showed no such effect. It is possible to induce apoptosis of the allo-antigen specific T cells of grafts activated by allo-antigen by exogenous Fas ligand expressed on recipient cells and this might provide a new approach for preventing GVHD and IL-2 may be more suitable for clinical application.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Membrane Glycoproteins / T-Lymphocytes / Interferon-gamma / Interleukin-2 / Apoptosis / DNA, Complementary / Fas Receptor / Antigens, CD34 / Cell Biology Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2003 Type: Article
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Membrane Glycoproteins / T-Lymphocytes / Interferon-gamma / Interleukin-2 / Apoptosis / DNA, Complementary / Fas Receptor / Antigens, CD34 / Cell Biology Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2003 Type: Article