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Effects of AZT on leukemia cell line KG-1a proliferation and telomerase activity / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 277-281, 2012.
Article in Chinese | WPRIM | ID: wpr-330975
ABSTRACT
This study was purposed to investigate the effect of 3'-azido-2', 3'-dideoxythymidine (AZT)on the proliferation and telomerase activity of human acute myeloid leukemia cell line KG-1a. The effect of proliferation was detected by MTT assay after the KG-1a cell were stimulated for 24, 48 and 72 h with different concentrations of AZT; telomerase activity was detected with TRAP-PCR-ELISA assay; RT-PCR was used to detect telomerase hTERT mRNA expression. The results showed that the proliferation of KG-1a cells was inhibited in a time and concentration dependent manner after exposure to AZT for 24, 48 and 72 h; the KG-1a cells decreased in S phase and increased in G(2)/M phase with the increasing of the concentration of AZT; telomerase activity and hTERT-mRNA expression in the experimental groups decreased after treated with AZT, which was positively correlated with concentration of AZT. It is concluded that AZT inhibits KG-1a cell proliferation and induces apoptosis, which maybe related with its decreasing the telomerase activity and hTERT mRNA expression.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Zidovudine / Leukemia / Cell Cycle / Apoptosis / Telomerase / Cell Line, Tumor / Cell Proliferation / Metabolism Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Zidovudine / Leukemia / Cell Cycle / Apoptosis / Telomerase / Cell Line, Tumor / Cell Proliferation / Metabolism Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2012 Type: Article