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Regulatory mechanisms of PI3K/AKT signaling pathway in acute leukemia / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 18-21, 2012.
Article in Chinese | WPRIM | ID: wpr-331030
ABSTRACT
This study was aimed to analyze the expression profiles of PI3K/AKT signaling pathway genes from bone marrow samples of AML and ALL patients and normal samples. AML, ALL and normal bone marrow samples were collected from 6 AML, 6 ALL patients and 4 normal persons. The expression of PI3K/AKT signaling pathway genes including PTEN, CCND1, mTOR, RICTOR, FOXO1 were detected by real-time fluorescent quantification RT-PCR while GAPDH gene expression was used as an internal reference. The relative gene expression level was calculated by the method of the 2(-ΔΔCt). The results showed that the gene expression profiles were different between normal and leukemic groups. PTEN, mTOR and RICTOR expression levels were down-regulated, while FOXO1 and CCND1 levels were up-regulated in AML and ALL. PTEN was down-regulated in 10 out of the 12 samples; mTOR was down-regulated in 9 out of the 12 samples; RICTOR was down-regulated in 7 out of the 12 samples; FOXO1 was up-regulated in 9 out of the 12 samples and CCND1 was up-regulated in 7 out of the 12 samples. It is concluded that PI3K/AKT signal pathway is activated in both AML and ALL leukemic cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA, Messenger / Signal Transduction / Leukemia / Carrier Proteins / Case-Control Studies / Gene Expression Regulation, Leukemic / Cyclin D1 / PTEN Phosphohydrolase / Proto-Oncogene Proteins c-akt / Forkhead Transcription Factors Type of study: Observational study / Risk factors Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA, Messenger / Signal Transduction / Leukemia / Carrier Proteins / Case-Control Studies / Gene Expression Regulation, Leukemic / Cyclin D1 / PTEN Phosphohydrolase / Proto-Oncogene Proteins c-akt / Forkhead Transcription Factors Type of study: Observational study / Risk factors Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2012 Type: Article