Regulatory mechanisms of PI3K/AKT signaling pathway in acute leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 18-21, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-331030
ABSTRACT
This study was aimed to analyze the expression profiles of PI3K/AKT signaling pathway genes from bone marrow samples of AML and ALL patients and normal samples. AML, ALL and normal bone marrow samples were collected from 6 AML, 6 ALL patients and 4 normal persons. The expression of PI3K/AKT signaling pathway genes including PTEN, CCND1, mTOR, RICTOR, FOXO1 were detected by real-time fluorescent quantification RT-PCR while GAPDH gene expression was used as an internal reference. The relative gene expression level was calculated by the method of the 2(-ΔΔCt). The results showed that the gene expression profiles were different between normal and leukemic groups. PTEN, mTOR and RICTOR expression levels were down-regulated, while FOXO1 and CCND1 levels were up-regulated in AML and ALL. PTEN was down-regulated in 10 out of the 12 samples; mTOR was down-regulated in 9 out of the 12 samples; RICTOR was down-regulated in 7 out of the 12 samples; FOXO1 was up-regulated in 9 out of the 12 samples and CCND1 was up-regulated in 7 out of the 12 samples. It is concluded that PI3K/AKT signal pathway is activated in both AML and ALL leukemic cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Signal Transduction
/
Leukemia
/
Carrier Proteins
/
Case-Control Studies
/
Gene Expression Regulation, Leukemic
/
Cyclin D1
/
PTEN Phosphohydrolase
/
Proto-Oncogene Proteins c-akt
/
Forkhead Transcription Factors
Type of study:
Observational study
/
Risk factors
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2012
Type:
Article
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