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Application of reverse transcription-multiplex nested PCR to detect PDGFRB gene rearrangement in myeloproliferative disorders / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1443-1446, 2011.
Article in Chinese | WPRIM | ID: wpr-331058
ABSTRACT
In order to explore the value of reverse transcription(RT)-multiplex nested PCR for detecting PDGFRB gene rearrangement in myeloproliferative disorders (MPD), the PDGFRB rearrangement was detected qualitatively in 146 MPD cases by reverse transcription multiplex nested PCR. The results showed that 8 cases with PDGFRB fusion gene were found in 146 cases, the positive rate was 5.5%. Out of 8 cases with PDGFRB fusion gene, TEL-PDGRB fusion gene was found in 3 cases; HIP1-PDGFRB fusion gene in 2 cases; GIT2-PDGFRB, TP53BP1-PDGFRB and WDP48-PDGFRB fusion gene in 1 case, respectively. It is concluded that RT-multiplex nested PCR is a powerful tool for the detection of PDGFRB rearrangement, which helps to tentatively diagnose MPD and to provide the clues for targeting therapy.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Gene Rearrangement / Reverse Transcriptase Polymerase Chain Reaction / Receptor, Platelet-Derived Growth Factor beta / Diagnosis / Multiplex Polymerase Chain Reaction / Genetics / Methods / Myeloproliferative Disorders Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Gene Rearrangement / Reverse Transcriptase Polymerase Chain Reaction / Receptor, Platelet-Derived Growth Factor beta / Diagnosis / Multiplex Polymerase Chain Reaction / Genetics / Methods / Myeloproliferative Disorders Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2011 Type: Article