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High SIPA-1 expression in proximal tubules of human kidneys under pathological conditions / 华中科技大学学报(医学)(英德文版)
Article in English | WPRIM | ID: wpr-331107
Responsible library: WPRO
ABSTRACT
Systemic lupus erythematosus (SLE) and clear cell renal cell carcinoma (CC-RCC) are serious disorders and usually fatal, and always accompanied with pathological changes in the kidney. Signal-induced proliferation-associated protein 1 (SIPA-1) is a Rap1GTPase activating protein (Rap1GAP) expressed in the normal distal and collecting tubules of the murine kidney. Lupus-like autoimmune disease and leukemia have been observed in SIPA-1 deficient mice, suggesting a pathological relevance of SIPA-1 to SLE and carcinoma in human being. The expression pattern of SIPA-1 is as yet undefined and the pathogenesis of these diseases in humans remains elusive. In this study, we used both immunohistochemistry and quantum dot (QD)-based immunofluorescence staining to investigate the expression of SIPA-1 in renal specimens from SLE and CC-RCC patients. MTT assay and Western blotting were employed to evaluate the effects of SIPA-1 overexpression on the proliferation and apoptosis of renal cell lines. Semi-quantitative reverse transcriptase-PCR (RT-PCR) was applied to examine the changes of hypoxia-inducible factor-1α (HIF-1α) mRNA level. Results showed that SIPA-1 was highly expressed in the proximal and collecting tubules of nephrons in SLE patients compared to normal ones, and similar results were obtained in the specimens of CC-RCC patients. Although SIPA-1 overexpression did not affect cellular proliferation and apoptosis of both human 786-O renal cell carcinoma cells and rat NRK-52E renal epithelial cell lines, RT-PCR results showed that HIF-1α mRNA level was down-regulated by SIPA-1 overexpression in 786-O cells. These findings suggest that SIPA-1 may play critical roles in the pathological changes in kidney, and might provide a new biomarker to aid in the diagnosis of SLE and CC-RCC.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Humans / Nuclear Proteins / Base Sequence / Cell Line / Apoptosis / DNA Primers / Reverse Transcriptase Polymerase Chain Reaction / GTPase-Activating Proteins / Cell Proliferation Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Humans / Nuclear Proteins / Base Sequence / Cell Line / Apoptosis / DNA Primers / Reverse Transcriptase Polymerase Chain Reaction / GTPase-Activating Proteins / Cell Proliferation Language: English Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2015 Type: Article