Cross-talk between c-Jun/Ets1 involved in EB virus-encoded latent membrane protein 1 regulates expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 204-208, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-331191
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effect of AP-1 and Ets binding site adjacent to matrix metalloproteinase-9 (MMP-9) promoter on activation of MMP-9 transcription of nasopharyngeal carcinoma cells transfected with EBV-encoded latent membrane protein 1 (LMP1), and to ascertain if cross-talk between c-Jun and Ets1 is involved in LMP1-regulating expression of MMP-9.</p><p><b>METHODS</b>Site-directed mutagenesis technique was used to establish a series of mutants, including MMP-9-CAT-Ets(-540)mt, MMP-9-CAT-AP-1(-533)mt and MMP-9-CAT-AP-1(-533)/Ets(-540)mt. After the mutants were transfected into LMP1-expressing NPC HNE2 cells regulated by Tet-on system (pTet-on-LMP1 HNE2), CAT activity of these mutants were assayed with induction of LMP1. With blockade of c-Jun or Ets1 antisense oligonucleotides, the activity of MMP-9 induced by LMP1 was assayed with gelatin zymography.</p><p><b>RESULTS</b>The CAT activity of MMP-9-Ets(-540)mt-CAT, MMP-9-AP-1(-533)mt-CAT, MMP-9-AP-1(-533)/Ets(-540) mt-CAT decreased significantly compared to MMP-9-CAT wt. After blockade with c-Jun or Ets1 antisense oligonucleotides, activity of MMP-9 induced by LMP1 decreased significantly, especially with combined blockade of c-Jun and Ets1.</p><p><b>CONCLUSION</b>The results suggest that transcription factor AP-1 and Ets play an crucial role in activation of MMP-9 transcription induced by LMP1, and cross-talk between c-Jun/Ets1 is involved in expression of MMP-9 mediated by LMP1.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Tumor Cells, Cultured
/
Transfection
/
Viral Matrix Proteins
/
Nasopharyngeal Neoplasms
/
Proto-Oncogene Proteins c-jun
/
Herpesvirus 4, Human
/
Matrix Metalloproteinase 9
/
Proto-Oncogene Protein c-ets-1
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2005
Type:
Article
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