Screening of differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 138-140, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-331208
ABSTRACT
<p><b>OBJECTIVE</b>To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.</p><p><b>METHODS</b>A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastasis Hca-F and its synogenetic cell line Hca-P with low metastatic potential was constructed by suppression subtractive hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed.</p><p><b>RESULTS</b>Ten differentially expressed cDNA fragments of Hca-F with high potential of lymphatic spreading were obtained, two of which were newly identified ones.</p><p><b>CONCLUSION</b>SSH is a useful technique to detect genes of differential expression and an effective method to clone novel genes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
RNA, Messenger
/
Gene Expression Regulation, Neoplastic
/
Gene Library
/
Carcinoma, Hepatocellular
/
Gene Expression Profiling
/
Cell Line, Tumor
/
Genetics
/
Liver Neoplasms, Experimental
/
Lymphatic Metastasis
Type of study:
Diagnostic study
/
Screening study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2005
Type:
Article
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