Fluorescence spectra and test application study on the interaction of fluorescein and bovine serum albumin / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1325-1327, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-331421
ABSTRACT
A fluorescence method is found for determination of bovine serum albumin (BSA). The method is based on the interaction of fluorescein with BSA to form a complex in Tris-HCl buffer of pH 2.2. The complex exitation wavelength is 467 nm, the emission wavelength is 515 nm. The linear measurement rang is between 1.8-500 mg/L for BSA, F = 26.776 C + 2.8082, r = 0.9999. This method is sensitive,steady,and low cost for determination of BSA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spectrometry, Fluorescence
/
Serum Albumin, Bovine
/
Chemistry
/
Fluorescein
/
Drug Interactions
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2006
Type:
Article
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