Construction of the recombinant integrating shuttle plasmid with cfpl0-esat6 fusion gene of Mycobacterium tuberculosis and its expression in BCG / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1298-1302, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-331426
ABSTRACT
This study was conducted to amplify the cfpl0-esat6 fusion gene by SOE and insert into the integrating shuttle plasmid pMV361 to form the recombinant plasmid. Then another recombinant plasmid was constructed by insertinga-A g signal sequence of BCG. The two recombinant plasmids were introduced into BCG and the induced products from recombinant BCG were analyzed. In conclusion,the successful construction of rBCG expressing the fusion protein CFP10-ESAT6 will be the base of the development of novel Mycobacterium tuberclosis vaccines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Bacterial Proteins
/
Recombinant Fusion Proteins
/
Tuberculosis Vaccines
/
Allergy and Immunology
/
Genetics
/
Metabolism
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
/
Antigens, Bacterial
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2006
Type:
Article
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