Characteristics of Nicotinic Receptor Expressed in Human Retinoblastoma
Journal of the Korean Ophthalmological Society
;
: 1060-1067, 2005.
Article
in Korean
| WPRIM
| ID: wpr-33153
ABSTRACT
PURPOSE:
To identify the characteristics and physiological function of the nicotinic receptor expressed in human retinoblastoma cells.METHODS:
We measured possible nicotinic signaling in WERI-Rb-1 cells using the Ca2+ imaging technique and the patch clamp method.RESULTS:
1) Nicotine-induced [Ca2+]i rise arose entirely through Ca2+ influx, which was completely abolished by hexamethonium (100 micro M). 2) Nicotine also induced remarkable depolarization from -56.6 +/- 3.7 mV to -29.6 +/- 3.6 mV (n=4) under current clamp mode, but it failed to directly activate the T-type Ca2+ channel expressed in retinoblastoma cells.CONCLUSIONS:
Nicotinic activation can increase the intracellular calcium level through calcium influx in the undifferentiated retinoblastoma cells, which may play important roles in cell proliferation, differentiation, and cell death.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retinoblastoma
/
Calcium
/
Receptors, Nicotinic
/
Cell Death
/
Hexamethonium
/
Cell Proliferation
/
Nicotine
Type of study:
Prognostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2005
Type:
Article
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