Amelioration of insulin resistance after scald by c-Jun N-terminal kinase inhibitor in rat / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 466-468, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-331542
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role and mechanism of c-Jun N-terminal kinase (JNk) inhibitor (SP600125) in amelioration of insulin resistance after scald.</p><p><b>METHODS</b>Twenty-four Sprague-Dawley rats were randomized into sham (the process of scald was mimicked by water at room temperature) , scald, scald and SP600125 groups. The rats were inflicted with 30% TBSA full-thickness scald in the latter two groups. Euglycemic-hyperinsulinemic glucose clamp experiment was carried out 4 days after scald. SP600125 was administered to the rats in scald and SP600125 2 hrs before Euglycemic-hyperinsulinemic glucose clamp was performed. Changes in the phospho-Serine307 and phospho-tyrosine of IRS-1 activity, as well as expression of phospho-JNK in muscles were determined.</p><p><b>RESULTS</b>Euglycemic-Hyperinsulinemic Glucose Clamps experiment showed that the infusion rate of 100 g/L glucose in sham, scald, scald and SP600125 groups were (12. 33 +/-0. 42) , (6. 61 +/-0. 27) , (11. 11 +/-0. 68) mgx kg(-1) x min(-1) , respectively ( P <0.01). The level of IRS-1 Serine307 phosphorylation and JNK activity in muscles were significantly increased, while insulin-induced tyrosine phosphorylation of IRS-1 decreased markedly after scald. Compared with scald group, the level of IRS-1 Serine307 phosphorylation and JNK activity in scald and SP600125 group were decreased but tyrosine phosphorylation was elevated.</p><p><b>CONCLUSION</b>SP600125 can partially ameliorate insulin resistance after scald by inhibition of JNK activation, and decrease the level of IRS-1 phospho-serine307.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphorylation
/
Burns
/
Insulin Resistance
/
Rats, Sprague-Dawley
/
JNK Mitogen-Activated Protein Kinases
/
Protein Kinase Inhibitors
/
Insulin Receptor Substrate Proteins
/
Hyperinsulinism
/
Insulin
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2006
Type:
Article
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